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内容简介:
Completely updated and in full color, this 3rd edition covers all the essentials of diagnostic microbiology. A logical building-block approach supplies what students need to know in an easy-to-use, memorable format. Material is presented in a progressive manner, from basic principles and concepts to systematic identification of etiologic agents of infectious diseases, promoting greater understanding and the development of problem-solving skills. Part I of the text explains basic principles and concepts, setting up a firm foundation in microbiology. Building on these basics, Part II highlights methods for the identification of significant isolates. Part III uses an organ system approach to discuss the laboratory diagnosis of infectious diseases with a focus on the most medically significant and commonly encountered diseases.
More than 800 full-color illustrations.
Hands-on procedures describe laboratory techniques.
A case study begins each chapter with a related, real-life situation.
All chapters make learning easier with features such as outlines, objectives, key terms, chapter summaries, points to remember, and learning assessment questions.
Five new chapters:
The Laboratory Role in Infection Control
Biochemical Identification of Gram-Negative Bacteria
Immunodiagnosis of Infectious Diseases
Agents of Bioterror
Biofilms: Architects of Disease
Antimicrobial Susceptibility Testing chapter has been expanded to include emerging microbial resistance and pharmacokinetic and pharmacodynamic principles and applications.
Issues to Consider encourages critical thinking and analysis of featured case studies.
Glossary of terms serves as a quick reference.
New co-editor, Don Lehman, brings years of educational experience.
书籍目录:
PART Ⅰ INTRODUCTION TO CLINICAL MICROBIOLOGY
1 Bacterial Cell StructurePhysiologyMetabolismand Genetics
Significance
Overview of the Microbial World
Classification/Taxonomy
Comparison of Eukaryotic and Prokaryotic Cell Structure
Bacterial Morphology
Microbial Growth and Nutrition
Bacterial Biochemistry and Metabolism
Bacterial Genetics
2 Host-Pathogen Interaction
A. The Role of the Usual Microbial Flora
Origin of Microbial Flora
Composition of the Microbial Flora at Different Body Sites
Role of the Microbial Flora in the Pathogenesis of Infectious Disease
Role of the Microbial Flora in the Host Defense against Infectious Disease
B. Pathogenesis of Infection
Microbial Factors Contributing to Pathogenesis and Virulence
Host Resistance Factors
Mechanisms by which Microbes May Overcome the Host Defenses Routes of Transmission
3 The Laboratory Role in Infection Control
General Concepts in Infection Control Practice
Outbreak Investigation
Education
Emerging and Reemerging Pathogens
4 Control of Microorganisms
A. Disinfection and Sterilization
Sterilization versus Disinfection
Factors That Influence the Degree of Killing
Methods of Disinfection and Sterilization
Disinfectants versus Antiseptics
EPA Regulations on Chemical Surface Disinfectants
FDA Regulations on Chemical Skin Antiseptics
B. Microbiology Safety
General Microbiology Safety
Bioterrorism and the Clinical Microbiology Laboratory
5 Performance improvement in the Microbiology Laboratory
A. Quality Issues in Clinical Microbiology
General Guidelines for Establishing Quality Control Performance Improvement
B. Putting the Laboratory Test to the Test
Analytical Analysis of Tests
Clinical Analysis of Tests
Operational Analysis of Tests
Choosing a Laboratory Method
Test Validation
6 Specimen Collection and Processing
Basic Principles of Specimen Collection
PreservationStorageand Transport of Specimens
Specimen Receipt and Processing
Culture Workup
Nonroutine Specimens
Communication of Laboratory Findings
7 Microscopic Examination of Infected Materials
Preparation of Samples
Stains
Microscopes
Terminology for Direct Examinations
Examination of Prepared Material
Grading or Classifying Materials
Reports of Direct Examinations
Examples of Sample Observations and Reports
Quality Control in Direct Microscopic Interpretations
8 Use of Colonial Morphology for the Presumptive Identification of Microorganisms
Importance of Colonial Morphology as a Diagnostic Tool
Initial Observation and Interpretation of Cultures
Gross Colony Characteristics Used to Differentiate and Presumptively Identify Microorganisms
Colonies with Multiple Characteristics
Growth of Organisms in Liquid Media
……
PART Ⅱ LABORATORY IDENTIFICATION OF SIGNIFICANT ISOLATES
PART Ⅲ LABORATORY OIAGNOSIS OF INFECTIOUS: AN ORGAN SYSTEM APPROACH
APPENDIXES
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The Univ. of Texas Health Science Center, San Antonio, TX. Covers the essentials of diagnostic microbiology. More that 650 illustrations, mostly in color, explain concepts. Key terms, questions, case studies, and increased coverage of emerging pathogens. Previous edition: c1995. DNLM: Microbiological Techniques. --This text refers to an out of print or unavailable edition of this title.
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